| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.026327 |
| Chromosome: | chromosome 12 |
| Location: | 1805834 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512700 | (1 of 1) PTHR12716:SF8 - TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGGCCAAGAGGCTGGCCATAGGGTTTTGGTATGATGGGAGGCAACAGT |
| Internal bar code: | TGGGAATGGTTTCATTAAGTGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 657 |
| LEAP-Seq percent confirming: | 13.6364 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGATTCACGTAACTGCGCAG |
| Suggested primer 2: | CTCTGCAGACCCCAGAACTG |