Insertion junction: CLIP2.026947_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.026947
Chromosome:chromosome 15
Location:458494
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g635800 SMC1 Structural Maintenance of Chromosomes protein; (1 of 1) K06636 - structural maintenance of chromosome 1 (SMC1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGCCCTGACAATGGGAGAGATGCCATCATGGGATGGACGGGTCTTTGGA
Internal bar code:TGATGGTAGCTTGTTAGTAAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:1098
LEAP-Seq percent confirming:97.0588
LEAP-Seq n confirming:33
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:34

Suggested primers for confirmation by PCR

Suggested primer 1:AAACCAACGAAAACGAGCGG
Suggested primer 2:GCATCTCCCTTGCCTTTCCT