| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.027109 |
| Chromosome: | chromosome 10 |
| Location: | 5827774 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g460466 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACACACACACACACACACACACACACACACACACACACACACACACAC |
| Internal bar code: | ATTCGGCTTGTAAATGTGTATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3384 |
| LEAP-Seq percent confirming: | 97.2603 |
| LEAP-Seq n confirming: | 71 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCGAACCCAATGCAGATGT |
| Suggested primer 2: | AAAAGGAAACGAAAGCCCGC |