Insertion junction: CLIP2.027221_3

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.027221
Chromosome:chromosome 13
Location:2931643
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g583550 VIP1,VIPP1 (1 of 2) K03969 - phage shock protein A (pspA); Vesicle inducing protein in plastids 1 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGGTGAAGTTAAATCATCTGTGTTGGCAGTGGTACCCACAGCGGGGGC
Internal bar code:TGTCGACGTTTTATTTGCCTAA

Confirmation - LEAP-Seq

LEAP-Seq distance:865
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:GTCACCCTGTCAACGTTTGC
Suggested primer 2:TGGGAAGACGTAGGTAGGCA