Insertion junction: CLIP2.028557_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.028557
Chromosome:chromosome 6
Location:4063097
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g278221 TSSP1,TPS1,TSPSP1 (1 of 1) 2.4.1.15//3.1.3.12 - Alpha,alpha-trehalose-phosphate synthase (UDP-forming) / UDP-glucose--glucose-phosphate glucosyltransferase // Trehalose-phosphatase / Trehalose 6-phosphate phosphatase; Trehalose-6-phosphate synthase/phosphatase, class I intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTGCAAACCCACCACTCGACCCCCACTCACCCGACACCACCAGCATGC
Internal bar code:GAGTTCTGGGGATGGTACATAT

Confirmation - LEAP-Seq

LEAP-Seq distance:556
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:TCCATGCAGATCACCAACCC
Suggested primer 2:GTTGGTGAGGGAGTGGTGAG