| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.028648 |
| Chromosome: | chromosome 7 |
| Location: | 2117031 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g326450 | VPS5B | (1 of 2) K17917 - sorting nexin-1/2 (SNX1_2); Subunit of Retromer complex | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTGACGTGAGCGGGCGCCCGGGCGGCGCGGTCTGGGTCAAGGGTGGCG |
| Internal bar code: | GGGGATATATCCGCTGTTATGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1827 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGATGTAGCCGCGGTAGTG |
| Suggested primer 2: | CGTTGGCGAAGACGATGTTC |