| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.029039 |
| Chromosome: | chromosome 12 |
| Location: | 9210751 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g542950 | CGL137,NUP54 | Nucleoporin 54; (1 of 1) K14308 - nuclear pore complex protein Nup54 (NUP54, NUP57) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGGGACGGAGCTTGGTGGTAGGCCGTGGTGGAGGGAGTAAGGGCACG |
| Internal bar code: | ATAACAATTACTGTGGGTAGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 827 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 15 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGTTGTATGACATGCGCG |
| Suggested primer 2: | TTACGCTGAGTGGGGCATTT |