Insertion junction: CLIP2.029298_2

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.029298
Chromosome:chromosome 2
Location:7030306
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g389763 HPAT4 Hydroxyproline O-arabinosyl transferase 4 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCGCGCCCAAACCTGTCCCCACCACGTACCCGAAACGCTATGTGAGCTAG
Internal bar code:TACTCCTGGAGGGCGATGTGTA

Confirmation - LEAP-Seq

LEAP-Seq distance:4809
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:103
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:103

Suggested primers for confirmation by PCR

Suggested primer 1:GGGCGAAGAAGACCAACTCA
Suggested primer 2:CGACTTATCCGACTGCTGCT