| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.029474 |
| Chromosome: | chromosome 5 |
| Location: | 1243691 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g241635 | (1 of 1) IPR012344//IPR014001//IPR027417 - Matrix protein, lentiviral and alpha-retroviral // Helicase superfamily 1/2, ATP-binding domain // P-loop containing nucleoside triphosphate hydrolase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCGCACATCCCTGCAAGCAAACGCAGGAGCAAACGCGATGCGATGCATT |
| Internal bar code: | CGGAGGTTTTGGTAGTACCTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3379 |
| LEAP-Seq percent confirming: | 89.1304 |
| LEAP-Seq n confirming: | 41 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTACAACTGTGTCCACGA |
| Suggested primer 2: | TGCCAGAGCTTCTGCAATCA |