| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.029500 |
| Chromosome: | chromosome 2 |
| Location: | 8065002 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g144550 | (1 of 4) PTHR10110:SF119 - SODIUM/HYDROGEN EXCHANGER 5-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTTTGAACTGTGAGTGTGCTGGCATCAGGTCGTGCGGTGTGGTGGGCCGC |
| Internal bar code: | GTGACTCCGATGGGGGATCACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1354 |
| LEAP-Seq percent confirming: | 83.871 |
| LEAP-Seq n confirming: | 26 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGATGAAAGCGTCGCATGAC |
| Suggested primer 2: | GCAACCACTAATAGCGCTGC |