Insertion junction: CLIP2.030405_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.030405
Chromosome:chromosome 13
Location:2259260
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre13.g578501 (1 of 1) IPR009060//IPR015940//IPR026741//IPR026937//IPR027417 - UBA-like // Ubiquitin-associated domain // Protein strawberry notch // Strawberry notch, helicase C domain // P-loop containing nucleoside triphosphate hydrolase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGTCATCGCGATCGTGCGACAGGCTACGAGGCGTTTGACGCGCTAGTG
Internal bar code:GTGGGACCATTTTTACTTCAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:547
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:7
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AACCACACACAGGCGATGAT
Suggested primer 2:CCCGTTCCTGACAACTTCGA