Insertion junction: CLIP2.030714_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.030714
Chromosome:chromosome 2
Location:6420948
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388350 MMP11,RAT2 Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATAGGTCGGAGGCTGCTGAAGCGGAGCCTCCTGCAGGTACGACGGCGGC
Internal bar code:TTAAAGTCAAGTTCCGTGACAG

Confirmation - LEAP-Seq

LEAP-Seq distance:1357
LEAP-Seq percent confirming:94.4444
LEAP-Seq n confirming:17
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGGTAGCATCTGACCCA
Suggested primer 2:CTTGCGCTATGTGGTGAAGC