Insertion junction: CLIP2.030922_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.030922
Chromosome:chromosome 12
Location:5626407
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g531400 NPHP4,NPH4,POC10 (1 of 1) K16478 - nephrocystin-4 (NPHP4); Proteome of centriole protein 10 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCGTACGTTTGAGTTCCAGCTCAGCGTGTCGCTGTACGACGAGGGCTA
Internal bar code:TGTATTTTTGTTCTAGTGTACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1686
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:GTGACCAGGAAGTTCTCGGG
Suggested primer 2:CTCGTGTCCTTTGGCCTAGG