Insertion junction: CLIP2.030985_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.030985
Chromosome:chromosome 1
Location:5928029
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g042200 PBA1,PBAS1 Photosystem II Associated Subunit 1 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TATATGCCAGACAACCTTGACCAACGGTTCTCTGCGCGCCTTGAACCCCT
Internal bar code:CTATTTAAGTCTTAAAGGGGAC

Confirmation - LEAP-Seq

LEAP-Seq distance:367
LEAP-Seq percent confirming:3.0303
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:32
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:GACTCCTCTAGGTCGCATGC
Suggested primer 2:ACCACCACATCCTGCTTAGC