Insertion junction: CLIP2.031047_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.031047
Chromosome:chromosome 5
Location:1362556
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g234600 (1 of 100) IPR001810 - F-box domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACAATTGATATCGAGACCACCCAGCGTTCCGATGGTATAATAGCCGGAG
Internal bar code:TGTCGTTGGGGTGTTCGTGTTT

Confirmation - LEAP-Seq

LEAP-Seq distance:982
LEAP-Seq percent confirming:33.3333
LEAP-Seq n confirming:6
LEAP-Seq n nonconfirming:12
LEAP-Seq n unique pos:18

Suggested primers for confirmation by PCR

Suggested primer 1:CACACCCTTCCGTCTTCACA
Suggested primer 2:CGAAGGAACATGTTAGCGCG