| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.031047 |
| Chromosome: | chromosome 5 |
| Location: | 1362564 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g234600 | (1 of 100) IPR001810 - F-box domain | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATTGTAACGACGGGAAAAGGCCCTTCGAAGTTGTCACGACCGGGAAGT |
| Internal bar code: | TGTCGTTGGGGTGTTCGTGTTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1522 |
| LEAP-Seq percent confirming: | 85.1852 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 54 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAAGGAACATGTTAGCGCG |
| Suggested primer 2: | CACACCCTTCCGTCTTCACA |