| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.031106 |
| Chromosome: | chromosome 3 |
| Location: | 127958 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143867 | CDS | ||
| Cre03.g143887 | TSR1 | (1 of 1) 6.1.1.19 - Arginine--tRNA ligase / Arginyl-tRNA synthetase; Arginyl-tRNA Synthetase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTGGAAGCCCATTATTGAGGCGCAGAAGGACAGCGTTAAAGACAACAGC |
| Internal bar code: | GATGAAATTATCCAAAACGTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3860 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 77 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGTCCCGTGACGGTGATATC |
| Suggested primer 2: | CGGCACAGTGAAAACGTGTT |