Insertion junction: CLIP2.031242_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.031242
Chromosome:chromosome 10
Location:2969270
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g440300 SMC5A (1 of 2) PTHR19306//PTHR19306:SF1 - STRUCTURAL MAINTENANCE OF CHROMOSOMES 5,6 SMC5, SMC6 // STRUCTURAL MAINTENANCE OF CHROMOSOMES PROTEIN 5; Structural Maintenance of Chromosomes protein intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGAGGGAGCAAATAAACAACGGGCCGCAATCGGCATTACCTGGTCCAGT
Internal bar code:GTGTCGTACTCGTCGTGTATCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2876
LEAP-Seq percent confirming:94.7368
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CTACTCTCCGCCATGCTCTG
Suggested primer 2:AGAAGTGTCAAGTAGGCGGC