| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.031822 |
| Chromosome: | chromosome 12 |
| Location: | 3186093 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g500200 | MPA2 | Metallophosphoesterase/metallo-dependent phosphatase; (1 of 1) PTHR22953:SF18 - METALLOPHOSPHOESTERASE DOMAIN-CONTAINING PROTEIN | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCGGGTGAGCTAGTGCTTCTGAATGGGTCAAATGCGCCGTGCAGTGCAA |
| Internal bar code: | AGAGGTATAGGGCCATTCATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2003 |
| LEAP-Seq percent confirming: | 97.0149 |
| LEAP-Seq n confirming: | 65 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 67 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGACTCCAGGTGTGACAGGT |
| Suggested primer 2: | CATGAGACGGCCCTACCATC |