| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.031910 |
| Chromosome: | chromosome 12 |
| Location: | 5938078 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g533750 | (1 of 11) PF00632 - HECT-domain (ubiquitin-transferase) (HECT) | outside_mRNA | |
| Cre12.g533800 | CYN22 | Cyclophilin 22; (1 of 1) K09567 - peptidyl-prolyl isomerase H (cyclophilin H) (PPIH, CYPH) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGAGTGTGCGGCAGCGCCGCTGCTGCGGCCCGCAGCGCCTTGTCGTGGC |
| Internal bar code: | TGTCGCATGTTAAGGGGCCTGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3337 |
| LEAP-Seq percent confirming: | 95.8904 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 73 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCCAAAGACGGCTGAGAACT |
| Suggested primer 2: | GCACGCATACACGGTAACAC |