| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032057 |
| Chromosome: | chromosome 2 |
| Location: | 6432868 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g388400 | RAT1 | (1 of 1) PF07798 - Protein of unknown function (DUF1640) (DUF1640); Chloroplast tscA maturation factor | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAGCACTATGGAGAGGAGGCATGGCGACGGGGGGGAAACATGTATTCG |
| Internal bar code: | TTTACGTGCAGACCTACGGATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1351 |
| LEAP-Seq percent confirming: | 71.4286 |
| LEAP-Seq n confirming: | 10 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCGCAAAGCTGACCTTATGC |
| Suggested primer 2: | TCTTGTCCAGGACGTTCTGC |