Insertion junction: CLIP2.032421_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.032421
Chromosome:chromosome 10
Location:4624564
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g451600 SRR28,SRR28B (1 of 1) PF00059//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4); Lectin-domain glycosyl hydrolase CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACATGTTTTCCGGTGGCGACGCCGCCCATGGCCGCCTTGGCGCGGGGC
Internal bar code:AAATTGCAATTACGGCGGATAA

Confirmation - LEAP-Seq

LEAP-Seq distance:1832
LEAP-Seq percent confirming:93.9394
LEAP-Seq n confirming:31
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:33

Suggested primers for confirmation by PCR

Suggested primer 1:ATTCTCTTGCCACCGACCTG
Suggested primer 2:GGGCAGCACGTGATTTTGTT