Insertion junction: CLIP2.032483_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.032483
Chromosome:chromosome 5
Location:665360
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g245351 (1 of 1) PF00059//PF00530//PF00704//PF14295 - Lectin C-type domain (Lectin_C) // Scavenger receptor cysteine-rich domain (SRCR) // Glycosyl hydrolases family 18 (Glyco_hydro_18) // PAN domain (PAN_4) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GAGCACGCACGGACCTTCTGGAAATACAAAAGCATAGGCTACCCTCTCCT
Internal bar code:TGCGGCCGCCGCCAAGTAATTG

Confirmation - LEAP-Seq

LEAP-Seq distance:1558
LEAP-Seq percent confirming:92.1053
LEAP-Seq n confirming:35
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:38

Suggested primers for confirmation by PCR

Suggested primer 1:CATGCAGCGTCACAATAGGC
Suggested primer 2:TGTAGTGGCCGAGACGTCTA