| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.032546 |
| Chromosome: | chromosome 1 |
| Location: | 5253274 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g036850 | PDY1 | (1 of 2) 1.5.5.2 - Proline dehydrogenase; Proline dehydrogenase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCTTGTGTGTGATGCAGAGCTCTTGGGCTTAGCCACGGCGCACGTAGCCC |
| Internal bar code: | GGGTTGTCAACCGTTTGTTGAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3666 |
| LEAP-Seq percent confirming: | 86.0465 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 6 |
| LEAP-Seq n unique pos: | 43 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGGACCACCAAGCGATCTT |
| Suggested primer 2: | AGGAGCTCCTGCAGTACGTA |