Insertion junction: CLIP2.032548_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.032548
Chromosome:chromosome 6
Location:945092
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g256350 (1 of 3) 1.1.2.6 - Polyvinyl alcohol dehydrogenase (cytochrome) / PVADH intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTTTAGCGCGGACCGGGCTTACTTACTTGGCTGCCAGGGCGGCACACCG
Internal bar code:CCGCAACTAACCCTAGCAGGGC

Confirmation - LEAP-Seq

LEAP-Seq distance:2189
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:30
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:30

Suggested primers for confirmation by PCR

Suggested primer 1:CCAGGGTTTCGCAAACTTGG
Suggested primer 2:ACAAGAACTGGAGGAGTGCG