| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.032672 |
| Chromosome: | chromosome 11 |
| Location: | 370668 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g467579 | CSC6 | Chlamydomonadale-specific family C protein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGCGAACAGTATGCGGCCCAGGCCGCTGCGGTGTAGCAGCGGCACCACC |
| Internal bar code: | GTGATACCTCCTGTTAGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3798 |
| LEAP-Seq percent confirming: | 87.3786 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 103 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTCTGGCACCTGCTCCTT |
| Suggested primer 2: | ACCCACTGCTGCTGAGAATC |