Insertion junction: CLIP2.032900_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.032900
Chromosome:chromosome 15
Location:464042
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g635800 SMC1 Structural Maintenance of Chromosomes protein; (1 of 1) K06636 - structural maintenance of chromosome 1 (SMC1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGTGAAGTCGTAGGGGGGGGGAGGGTAGTGACCAATGGCGTGTGAAGGAG
Internal bar code:CGACGTACTCAAGTGCAATCAC

Confirmation - LEAP-Seq

LEAP-Seq distance:441
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:2
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AACGCAAATGGACAGGTTGC
Suggested primer 2:ATCACCACCCACTTGCTGAG