| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.033204 |
| Chromosome: | chromosome 2 |
| Location: | 6289975 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g387400 | POLZ1 | DNA polymerase zeta; (1 of 1) K02350 - DNA polymerase zeta (POLZ1, rev3) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCGTCACCGCAACTGGGTGCGCCGGTGCCGCCGCTGCCGCCACCCGTTGA |
| Internal bar code: | GTTCTGCCCTGAATAAAATGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2065 |
| LEAP-Seq percent confirming: | 93.3333 |
| LEAP-Seq n confirming: | 28 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 30 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATCTGGAGGAGGAAGGGGT |
| Suggested primer 2: | CTACACTGGCTTCTGCTGCT |