Insertion junction: CLIP2.033286_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.033286
Chromosome:chromosome 2
Location:2921719
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g095200 (1 of 3) PTHR19211:SF15 - ATP-BINDING CASSETTE SUB-FAMILY F MEMBER 2 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGCCACGTGACATGAAGGTACGCCTGGTACGGCTGGCGGTGTGAAGGCCG
Internal bar code:AAAGGGTGTTTAAAAATGTAGA

Confirmation - LEAP-Seq

LEAP-Seq distance:4276
LEAP-Seq percent confirming:95.4545
LEAP-Seq n confirming:21
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:CCTACCACTCTCCACGCATC
Suggested primer 2:ATCCAACAAACGCACAGCAC