| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.033294 |
| Chromosome: | chromosome 12 |
| Location: | 1805534 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512700 | (1 of 1) PTHR12716:SF8 - TRANSCRIPTION INITIATION FACTOR IIE SUBUNIT BETA | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAACGGCAGCATGCAACAAGTGGGTTTCACCGAACACACCCGCGTGCTGT |
| Internal bar code: | TGAACCGCCCGATATCAGCTAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2568 |
| LEAP-Seq percent confirming: | 95.6522 |
| LEAP-Seq n confirming: | 44 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 46 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACCCTATGGCCAGCCTC |
| Suggested primer 2: | CAAGCAGCAGGACCTACACA |