Insertion junction: CLIP2.033365_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.033365
Chromosome:chromosome 15
Location:465236
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g635800 SMC1 Structural Maintenance of Chromosomes protein; (1 of 1) K06636 - structural maintenance of chromosome 1 (SMC1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCAGCCCAAACTGAACCAAACCCAATGCAAAAGGACGAGGGGAGAGAG
Internal bar code:TATTGGCAGGGGCAATCCCGGG

Confirmation - LEAP-Seq

LEAP-Seq distance:508
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:AGCAAGTGGGTGGTGATGAG
Suggested primer 2:GCAAGCTTTCGGTCGAATCC