Insertion junction: CLIP2.033714_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.033714
Chromosome:chromosome 3
Location:6755248
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g196450 (1 of 1) K15178 - RNA polymerase-associated protein RTF1 (RTF1) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AACTTCCACAAGACTTGAGCATGTGGCAGTATAGCCACCTAACCGGCTAC
Internal bar code:GACTAGTAGGGAGTCTAACATG

Confirmation - LEAP-Seq

LEAP-Seq distance:2776
LEAP-Seq percent confirming:96.8254
LEAP-Seq n confirming:61
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:63

Suggested primers for confirmation by PCR

Suggested primer 1:CTGACGACCTAGACGATGCC
Suggested primer 2:TCTGCCTCCTTAGCCTTTGC