Insertion junction: CLIP2.033729_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.033729
Chromosome:chromosome 12
Location:3664585
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g513500 XAB2 (1 of 1) K12867 - pre-mRNA-splicing factor SYF1 (SYF1, XAB2); Transcription-coupled DNA repair protein, possible splicing factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACTCCTGGAGGGGGGGAGGGGAGGACATTGGACGGAGACGGGGTGACGAC
Internal bar code:AGTAGGCGACCATGATATTGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:2392
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:36
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:36

Suggested primers for confirmation by PCR

Suggested primer 1:CTGCGCTCGTATCACTTTGC
Suggested primer 2:TTTCATGCTTTGGCCACACG