| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.033826 |
| Chromosome: | chromosome 2 |
| Location: | 3208523 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g096900 | TIE1 | (1 of 2) 4.6.1.16 - tRNA-intron lyase / tRNA splicing endonuclease; tRNA-intron endonuclease | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGAGCCGGGCAAAGCGTATGCCCGCGCCGTGCCCATTGGCAGGTATG |
| Internal bar code: | TGAGCGCAAATGGTTGTACTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1631 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 18 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTTCCTTTGCAGCTTTGCT |
| Suggested primer 2: | CCGTGTGTCCTGACTCGAAA |