Insertion junction: CLIP2.034230_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.034230
Chromosome:chromosome 2
Location:6420951
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388350 MMP11,RAT2 Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTCGGAGGCTGCTGAAGCGGAGCCTCCTGCAGGTACGACGGCGGCTGG
Internal bar code:TAACACGATTATGGCCGATTAT

Confirmation - LEAP-Seq

LEAP-Seq distance:1643
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:9
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:9

Suggested primers for confirmation by PCR

Suggested primer 1:TACCGGTAGCATCTGACCCA
Suggested primer 2:CTTGCGCTATGTGGTGAAGC