| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.034395 |
| Chromosome: | chromosome 5 |
| Location: | 3006103 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g230850 | (1 of 1) IPR001374//IPR002110//IPR020683 - R3H domain // Ankyrin repeat // Ankyrin repeat-containing domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACGTTGTACAAGTGGGCCCGCGACGCAGGTGGGGGCCCAGGTGGCGTGCG |
| Internal bar code: | GTGCGTGCGAATATTAGTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 872 |
| LEAP-Seq percent confirming: | 88.8889 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGAGCGAATTATGCGGA |
| Suggested primer 2: | ATGGGGGCAAATGGATTCGT |