| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.034547 |
| Chromosome: | chromosome 2 |
| Location: | 7350741 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391801 | VSP4 | (1 of 8) PTHR11339:SF290 - PROTEIN T26A8.1; Hydroxyproline-rich cell wall glycoprotein | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAGTCCGGCGAAATCTGTGGACATGTTCAGGAGCGCCGGGGACTGGT |
| Internal bar code: | GCAAAGGGAATCACGTCAATTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3226 |
| LEAP-Seq percent confirming: | 99.1071 |
| LEAP-Seq n confirming: | 111 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 112 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTAGTATGCTGCGAGCGTC |
| Suggested primer 2: | GAGCATGACGTGGAAGACCA |