| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.034852 |
| Chromosome: | chromosome 4 |
| Location: | 2209964 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre04.g220200 | (1 of 1) PTHR16254:SF13 - K(+) EFFLUX ANTIPORTER 1, CHLOROPLASTIC-RELATED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTTCAACACCTGGGATGTGTGTGTGTTTGTCAGCTGCGCATCAGTGTGC |
| Internal bar code: | TGGGCGAATCTCGCGGGAGGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 303 |
| LEAP-Seq percent confirming: | 32.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 17 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGGGAGAGGTAGGAAAGGT |
| Suggested primer 2: | CATCCCATTAAGCACGCGTG |