| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.035409 |
| Chromosome: | chromosome 6 |
| Location: | 8886183 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g311850 | HAD1 | Halo-acid dehalogenase-like hydrolase; (1 of 1) PTHR18901//PTHR18901:SF27 - 2-DEOXYGLUCOSE-6-PHOSPHATE PHOSPHATASE 2 // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGAAGCAACACAGGGAAGCAAGTGGGGCGTGGTATGACGTGGATGAAGG |
| Internal bar code: | TTGATTCTGCTGGTTAGTAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 5256 |
| LEAP-Seq percent confirming: | 98.3607 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 61 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TACACACCTCTTGCGACACC |
| Suggested primer 2: | GGCGCGGCAGTAATAAAGTG |