Insertion junction: CLIP2.035455_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.035455
Chromosome:chromosome 4
Location:1871979
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre04.g218150 PPH1,PPP18 Thylakoid phosphoprotein phosphatase 2C-related; (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TACCCATCACCCCTTCCCCCCACCTCCCTCCCCTTCGCAACCCTACACCT
Internal bar code:GAGGGCTCTTAGGTCAAAGACA

Confirmation - LEAP-Seq

LEAP-Seq distance:1618
LEAP-Seq percent confirming:83.3333
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:CGCGCTAACATGTTCCTTCG
Suggested primer 2:CTGTAGCGGCTCGACATCAT