| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.035591 |
| Chromosome: | chromosome 16 |
| Location: | 5181115 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g685000 | (1 of 3) PTHR13748//PTHR13748:SF43 - COBW-RELATED // SUBFAMILY NOT NAMED | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAACAACGCACGCAGACTTAGCAGGGTTGGTCACGCAAAACGGTAACATG |
| Internal bar code: | GAACTATATAAAGTGCTATGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1549 |
| LEAP-Seq percent confirming: | 85.7143 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 28 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATTGCTGCTCGAATGCCTG |
| Suggested primer 2: | CTTGCGTCATGTGCCACAAA |