Insertion junction: CLIP2.036092_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.036092
Chromosome:chromosome 1
Location:816579
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g004550 FAP190 (1 of 7) 2.7.10.2//2.7.12.1 - Non-specific protein-tyrosine kinase / Cytoplasmic protein tyrosine kinase // Dual-specificity kinase; Flagellar Associated Protein 190 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCGCGGCGCTGCGCGGCTTGCTGGCGGGGGGCTTGTCTGAGAGGCCCGC
Internal bar code:CGTTTTGTTCGACATATGGGTG

Confirmation - LEAP-Seq

LEAP-Seq distance:3586
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:127
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:127

Suggested primers for confirmation by PCR

Suggested primer 1:AACCTGCCTTGTGCGTATCA
Suggested primer 2:ATACTGCTGGTGGACTTGCC