Insertion junction: CLIP2.036109_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.036109
Chromosome:chromosome 2
Location:6423591
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g388350 MMP11,RAT2 Metalloproteinase of VMP family; (1 of 51) PF05548 - Gametolysin peptidase M11 (Peptidase_M11) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGGGAGGAGACCAGGTGCGGGGCGTGCGTGGGGTGCGCTGCGTGCGTGAG
Internal bar code:TTTGATTGGGAACTAATTGGTC

Confirmation - LEAP-Seq

LEAP-Seq distance:1718
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:11
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:11

Suggested primers for confirmation by PCR

Suggested primer 1:CCATGAAGGTGGAGGTGTCC
Suggested primer 2:CCGGCGTTTGTTTTCGAGTT