Insertion junction: CLIP2.036478_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:-
Strain:CLIP2.036478
Chromosome:chromosome 1
Location:5641154
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g040350 MSH8 putative MutS type 2 DNA repair protein; (1 of 2) PTHR11361//PTHR11361:SF14 - DNA MISMATCH REPAIR MUTS RELATED PROTEINS // DNA MISMATCH REPAIR PROTEIN MUTS2 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CCCGCTGCCCCGCTTCCCCCACGTGTCCCCGCTCTCCCTCACCCCCTTCA
Internal bar code:ATGAGTGAAACATGGTGTTTGA

Confirmation - LEAP-Seq

LEAP-Seq distance:108
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CGTCATCAATGCCATGCCAG
Suggested primer 2:CCTGCACTGACCTGCACTAA