Insertion junction: CLIP2.036507_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.036507
Chromosome:chromosome 8
Location:560353
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre08.g359650 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGAAACAGTCGCGCTGCAAGGAGAAACTGCAGCCGCATGGTTGGGGGGT
Internal bar code:GGGTCACTTTAATACCTAATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:110
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:4

Suggested primers for confirmation by PCR

Suggested primer 1:CGGGACCCATGTGTGTACAA
Suggested primer 2:ATCACAACAGCAACAACCGC