| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.036528 |
| Chromosome: | chromosome 10 |
| Location: | 4778589 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g452850 | intron | ||
| Cre10.g452900 | SMY2 | (1 of 2) 3.1.4.12 - Sphingomyelin phosphodiesterase / Neutral sphingomyelinase; Sphingomyelin phosphodiesterase/sphingomylinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTTCACATGCGCCGAGAGTTTGCACGCACCAGCTCCTTCAGCTCCACC |
| Internal bar code: | ACTTGCGCGGAGGTAAGACACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 910 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 25 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 25 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGCAGGCTTGAGTCTTGGAT |
| Suggested primer 2: | TCCTGCGCAAACCAATTTGG |