| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | - |
| Strain: | CLIP2.036548 |
| Chromosome: | chromosome 10 |
| Location: | 1576340 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g429000 | UBC39 | E2 ubiquitin conjugating enzyme; (1 of 1) IPR000104//IPR000608//IPR016135 - Antifreeze protein, type I // Ubiquitin-conjugating enzyme E2 // Ubiquitin-conjugating enzyme/RWD-like | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATCTTCTGGGGTCTGGGAATTACTGCACCCTACGGAAATTCACCAACCTG |
| Internal bar code: | ATCCGAGTGCTGTGCCGGATTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 187 |
| LEAP-Seq percent confirming: | 23.0769 |
| LEAP-Seq n confirming: | 3 |
| LEAP-Seq n nonconfirming: | 10 |
| LEAP-Seq n unique pos: | 13 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGCATATCAACGTGCAAGG |
| Suggested primer 2: | GGAGACATTTCGGCAGTTGC |