| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.036972 |
| Chromosome: | chromosome 1 |
| Location: | 5568006 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g039650 | PDF1C | (1 of 3) 3.5.1.88 - Peptide deformylase / Polypeptide deformylase; Peptide deformylase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAAGGTGCTGCAGGGCAACGGGGCAGCCGCAACGGGAGGCGGCACGATTG |
| Internal bar code: | GTTTTATGGCGTGAAGCCTTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 843 |
| LEAP-Seq percent confirming: | 80.0 |
| LEAP-Seq n confirming: | 8 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 10 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAGAGAAGGCCATCAGCACC |
| Suggested primer 2: | AACACCATCCCAGCCCTTTT |