| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.037711 |
| Chromosome: | chromosome 16 |
| Location: | 3657997 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g677026 | PDH1 | Pyruvate dehydrogenase E1 beta subunit; (1 of 2) K00162 - pyruvate dehydrogenase E1 component beta subunit (PDHB, pdhB) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATATCCTTGCAACCCCGCCCCGCGCCCCCCGCTCCTGCACCCACCTCGC |
| Internal bar code: | GGTTATCGCCGCTTAGTAGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1770 |
| LEAP-Seq percent confirming: | 91.6667 |
| LEAP-Seq n confirming: | 11 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGGTGTTACGTTCCCAGTCA |
| Suggested primer 2: | TGATGGAGCGCAGGTTGATT |