Insertion junction: CLIP2.038324_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.038324
Chromosome:chromosome 15
Location:3009779
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre15.g643503 HKR5,HKR6,HKR56,COP9,COP10 (1 of 1) IPR000104//IPR001054//IPR001789//IPR003594//IPR003661//IPR004358//IPR005467//IPR011006//IPR029730//IPR029787 - Antifreeze protein, type I // Adenylyl cyclase class-3/4/guanylyl cyclase // Signal transduction response regulator, receiver domain // Histidine kinase-like ATPase, C-terminal domain // Signal transduction histidine kinase, dimerisation/phosphoacceptor domain // Signal transduction histidine kinase-related protein, C-terminal // Histidine kinase domain // CheY-like superfamily // Archaeal/bacterial/fungal rhodopsin-like // Nucleotide cyclase; Histidine kinase rhodopsin 5/6 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CATGAATGTAACCCCCCCCCCCCACACACACACACACACACACCACCCTA
Internal bar code:ATTGGAGTCAGGCTAAGGCGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:178
LEAP-Seq percent confirming:13.0435
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:20
LEAP-Seq n unique pos:23

Suggested primers for confirmation by PCR

Suggested primer 1:GCGTCATCCTTCTCCCTAGC
Suggested primer 2:CCACAACACCCACAGCATTG